Beilstein J. Nanotechnol.2021,12, 242–256, doi:10.3762/bjnano.12.20
present a comprehensive characterization of the 3D nanomorphology of living, as well as fixed, osteoblastic cells using scanningionconductancemicroscopy (SICM), which is a nanoprobing method that largely avoids mechanical perturbations. Dynamic ruffles are observed, manifesting themselves in
capability of apical membrane features and fluctuation dynamics in aiding the assessment of adhesion and migration properties on a single-cell basis.
Keywords: cell adhesion; membrane fluctuations; osteoblast; plasma membrane nanomorphology; scanningionconductancemicroscopy (SICM); Introduction
remain obscure. A localized ion current flowing through a nanopipette probe represents a suitable non-invasive interaction, which is exploited in scanningionconductancemicroscopy (SICM) [19][20][21]. SICM is well suited to probe soft and responsive surfaces, such as those of living cells. The applied
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Figure 1:
SICM analysis of a fixed MG-63 osteoblastic cell. (a) Overview morphology of the cell on glass afte...